Journal: Frontiers in Chemistry

Article Title: Exposure to Mycotoxin-Mixtures via Breast Milk: An Ultra-Sensitive LC-MS/MS Biomonitoring Approach

doi: 10.3389/fchem.2020.00423

Figure Lengend Snippet: Optimized analyte specific MS and MS/MS parameters utilized on the QTrap6500 + instrument.

Article Snippet: Aflatoxin metabolites AFM 1 , AFM 2 , AFP 1 , AFQ 1 , AFB 1 -N7-guanine adduct, as well as AME, AOH, beauvericin (BEA), citrinin (CIT), HT-2 toxin, ochratoxin alpha (OTα), ochratoxin B (OTB) from Toronto Research Chemicals (Ontario, Canada).

Techniques:

Journal: Frontiers in Chemistry

Article Title: Exposure to Mycotoxin-Mixtures via Breast Milk: An Ultra-Sensitive LC-MS/MS Biomonitoring Approach

doi: 10.3389/fchem.2020.00423

Figure Lengend Snippet: Performance characteristics of the method as obtained during in-house validation on the QTrap6500 + instrument including concentration range of matrix matched standard calibration, regression coefficient ( R 2 ), spiking levels, recoveries of the extraction step (R E ), intermediate precision (RSD R ), repeatability (RSD r ), signal suppression/enhancement (SSE), limits of detection (LOD) and limits of quantification (LOQ).

Article Snippet: Aflatoxin metabolites AFM 1 , AFM 2 , AFP 1 , AFQ 1 , AFB 1 -N7-guanine adduct, as well as AME, AOH, beauvericin (BEA), citrinin (CIT), HT-2 toxin, ochratoxin alpha (OTα), ochratoxin B (OTB) from Toronto Research Chemicals (Ontario, Canada).

Techniques: Biomarker Discovery, Concentration Assay, Extraction

Journal: Frontiers in Chemistry

Article Title: Exposure to Mycotoxin-Mixtures via Breast Milk: An Ultra-Sensitive LC-MS/MS Biomonitoring Approach

doi: 10.3389/fchem.2020.00423

Figure Lengend Snippet: Comparison of limit of detection (LOD) values for all analytes using the published (Braun et al., 2018 ) and newly presented approach.

Article Snippet: Aflatoxin metabolites AFM 1 , AFM 2 , AFP 1 , AFQ 1 , AFB 1 -N7-guanine adduct, as well as AME, AOH, beauvericin (BEA), citrinin (CIT), HT-2 toxin, ochratoxin alpha (OTα), ochratoxin B (OTB) from Toronto Research Chemicals (Ontario, Canada).

Techniques: Comparison, Sample Prep

Journal: Frontiers in Pharmacology

Article Title: Decursinol-mediated antinociception and anti-allodynia in acute and neuropathic pain models in male mice: Tolerance and receptor profiling

doi: 10.3389/fphar.2022.968976

Figure Lengend Snippet: Serotonin Receptor 2 (5-HT 2 ) signaling does not attenuate decursinol-induced antinociception or hypothermia. Mediation of the acute antinociceptive (A/B) and hypothermic (C) effects of 50 mg/kg decursinol by 5-HT 2 antagonists in male mice. Mice (N = 10) were assessed for hot plate (A) and tail-flick (B) antinociception (%MPE) and hypothermia (C; %ΔBT) 30 min following pretreatment with either vehicle (VEH), 4 mg/kg of the non-selective 5-HT 2 antagonist Methysergide (MS), 4 mg/kg of the selective 5-HT 2A antagonist Volinanserin (5-HT 2A ), or 4 mg/kg of the selective 5-HT 2C receptor antagonist SB-242084 (5-HT 2C ) and again 30 min following treatment with either vehicle (VEH; unfilled bars) or 50 mg/kg of decursinol (DOH; filled bars). Error bars represents the mean ± SEM. Data were analyzed using a repeated measures one-way ANOVA with Bonferroni post-hoc tests (*** p < 0.001; **** p < 0.0001 compared to VEH/VEH; # p < 0.05 compared to VEH/DOH).

Article Snippet: Methysergide maleate, a non-selective 5-HT 2 receptor antagonist, was obtained from BOC Sciences (5281073; Shirley, NY) while the 5-HT 2A receptor antagonist volinanserin (5311271), the 5-HT 2C antagonist SB-242084 (3644637), the nonselective opioid antagonist naloxone hydrochloride (5464092), the CB 1 inverse agonist rimonabant hydrochloride (104849), and the CB 2 inverse agonist SR144528 (3081355) were all obtained from Cayman Chemical (Ann Arbor, MI).

Techniques: Tail Flick Test